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Shinya Yamanaka
The Nobel Prize in Physiology or Medicine 2012
Nobel co-recipient: Sir John B. Gurdon
Award: "for the discovery that mature cells can be reprogrammed to become pluripotent."
Patents
| Publication: | 1/37 |
| Publication No: | US 10385407 B2 |
| Title: | Method for screening induced pluripotent stem cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | August 20, 2019 |
| Filing Date: | March 1, 2019 |
| Inventors: | Shinya Yamanaka, Kazutoshi Takahashi, Michiyo Koyanagi, Mari Ohnuki |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 2/37 |
| Publication No: | US 10150949 B2 |
| Title: | Cardiomyocyte marker |
| Publication Type: | United States Utility Patent |
| Publication Date: | December 11, 2018 |
| Filing Date: | June 12, 2017 |
| Inventors: | Shinya Yamanaka, Yoshinori Yoshida, Shunsuke Funakoshi |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a method for producing or detecting cardiomyocytes by extracting/detecting cardiomyocytes from a cell population which includes cardiomyocytes using, as an index, positivity of NCAM1, SSEA3, SSEA4 and/or CD340. |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 3/37 |
| Publication No: | US 9404124 B2 |
| Title: | Method of producing induced pluripotent stem cells using inhibitors of P53 |
| Publication Type: | United States Utility Patent |
| Publication Date: | August 2, 2016 |
| Filing Date: | November 14, 2014 |
| Inventors: | Keisuke Okita, Masato Nakagawa, Shinya Yamanaka |
| Assignee: | Kyoto University |
| Abstract: | Provided is a method of producing an iPS cell, comprising bringing (a) Oct3/4 or a nucleic acid that encodes the same, (b) Klf4 or a nucleic acid that encodes the same, and (c) Sox2 or a nucleic acid that encodes the same, as well as (d1) L-Myc or a nucleic acid that encodes the same and/or (d2) a functional inhibitor of p53, into contact with a somatic cell. It is preferable that (a) a nucleic acid that encodes Oct3/4, (b) a nucleic acid that encodes Klf4, (c) a nucleic acid that encodes Sox2, (d1) a nucleic acid that encodes L-Myc and (e) a nucleic acid that encodes Lin28 or Lin28b be inserted into an episomal vector having loxP sequences placed in the same orientation on the 5′ and 3′ sides of a vector constituent essential for the replication of the vector, that (d2) a nucleic acid that encodes an shRNA against p53 be inserted into a vector ensuring transient expression (plasmid vector and the like), and that all these nucleic acids be transferred to a somatic cell. |
| Representative Figure: | |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 4/37 |
| Publication No: | US 10240126 B2 |
| Title: | Induced pluripotent stem cell selection method and method for inducing differentiation to blood cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | March 26, 2019 |
| Filing Date: | November 4, 2014 |
| Inventors: | Shinya Yamanaka, Yoshinori Yoshida, Masatoshi Nishizawa |
| Assignee: | Kyoto University |
| Abstract: | A method for producing hematopoietic stem cells and/or hematopoietic progenitor cells from pluripotent stem cells is described. The method includes a step of culturing pluripotent stem cells in the presence of IGF2. A method is described for selecting an induced pluripotent stem cell(s) having high capacity to differentiate into hematopoietic stem cells and/or hematopoietic progenitor cells, or into blood cells, based on the expression level(s) of one or more genes such as TRIM58, CTSF, FAM19A5, and TCERG1L genes, or on the DNA methylation state(s) of the TRIM58, CSMD1, and/or FAM19A5 gene(s). |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 5/37 |
| Publication No: | US 9822342 B2 |
| Title: | Method of efficiently inducing cardiomyocytes |
| Publication Type: | United States Utility Patent |
| Publication Date: | November 21, 2017 |
| Filing Date: | May 9, 2014 |
| Inventors: | WShinya Yamanaka, Yoshinori Yoshida, Kenji Miki |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a method for efficiently producing cardiomyocytes from pluripotent stem cells, which method comprises the steps of dissociating embryoid bodies obtained during the production process, and allowing reaggregation of the resulting cells to allow formation of embryoid bodies. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 6/37 |
| Publication No: | US 10077429 B2 |
| Title: | Method of efficiently establishing induced pluripotent stem cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | September 18, 2018 |
| Filing Date: | October 23, 2013 |
| Inventors: | Kazutoshi Takahashi, Koji Tanabe, Shinya Yamanaka |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a method of improving iPS cell establishment efficiency, comprising contacting a protein involved in primitive streak (PrS) formation, preferably Foxh1, or a nucleic acid that encodes the same with a somatic cell in a nuclear reprogramming step. Also provided is a method of producing an iPS cell, comprising contacting the protein involved in PrS formation or a nucleic acid that encodes the same, and nuclear reprogramming substance(s) with a somatic cell. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 7/37 |
| Publication No: | US 10519425 B2 |
| Title: | Highly efficient method for establishing induced pluripotent stem cell |
| Publication Type: | United States Utility Patent |
| Publication Date: | December 3, 2019 |
| Filing Date: | May 23, 2013 |
| Inventors: | Shinya Yamanaka, Keisuke Okita |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a production method of iPS cell, including a step of introducing the following (1) and (2): (1) an episomal vector containing a nuclear reprogramming factor; and (2) an episomal vector containing EBNA-1, which is different from (1), into a somatic cell, as well as a method for improving iPS cell establishment efficiency. The present invention also provides an agent for improving iPS cell establishment efficiency, which contains an episomal vector containing a nucleic acid encoding EBNA-1, and a kit for producing an iPS cell further containing an episomal vector containing a nucleic acid encoding a nuclear reprogramming factor. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 8/37 |
| Publication No: | US 9335323 B2 |
| Title: | Method for sorting of pluripotent cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | May 10, 2016 |
| Filing Date: | January 7, 2013 |
| Inventors: | Motonari Uesugi, Nao Hirata, Asako Murata, Young-Tae Chang, Norio Nakatsuji, Hirofumi Suemori, Eihachiro Kawase, Kaori Yamauchi, Kazumitsu Ueda, Yuto Fujibayashi, Shinya Yamanaka, Masato Nakagawa |
| Assignee: | Kyoto University |
| Abstract: | A method for sorting pluripotent cells using a compound which is eliminated from the pluripotent cells through the MDR1 transporter. |
| Representative Figure: | No Figure |
| Family | |
| Details | Google Patents USPTO Patent Database |
| Publication: | 9/37 |
| Publication No: | US 9499797 B2 |
| Title: | Method of making induced pluripotent stem cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | November 22, 2016 |
| Filing Date: | August 10, 2012 |
| Inventors: | Shinya Yamanaka, Keisuke Okita |
| Assignee: | Kyoto University |
| Abstract: | A method of producing an induced pluripotent stem cell includes introducing into a somatic cell one or more non-viral expression vectors. The vectors include one or more of an Oct family gene, a Klf family gene, a Sox family gene, a Myc family gene, a Lin family gene, and Nanog gene. The somatic cell is then cultured in a medium that supports pluripotent stem cells. At least a portion of the one or more introduced non-viral expression vectors is not substantially integrated in the chromosome.. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 10/37 |
| Publication No: | US 8791248 B2 |
| Title: | Nuclear reprogramming factor comprising miRNA and a protein factor |
| Publication Type: | United States Utility Patent |
| Publication Date: | July 29, 2014 |
| Filing Date: | December 7, 2011 |
| Inventors: | Shinya Yamanaka, Michiyo Koyanagi |
| Assignee: | Kyoto Univesity |
| Abstract: | A method of preparing induced pluripotent stem cells, comprising a nuclear reprogramming step with a nuclear reprogramming factor in the presence of miRNA, wherein said miRNA has a property of providing a higher nuclear reprogramming efficiency in the presence of said miRNA than in the absence thereof. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 11/37 |
| Publication No: | US 9506039 B2 |
| Title: | Efficient method for establishing induced pluripotent stem cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | November 29, 2016 |
| Filing Date: | December 2, 2011 |
| Inventors: | Shinya Yamanaka, Kazutoshi Takahashi, Koji Tanabe |
| Assignee: | Kyoto University |
| Abstract: | The invention provides a method of improving the efficiency of establishment of induced pluripotent stem cells by increasing, in a nuclear reprogramming step of somatic cell, the level of activated form of one or more proteins selected from the group consisting of Ras family members, PI3 kinase, RalGEF, Raf, AKT family members, Rheb, TCL1 and S6K. The invention also provides a method of producing induced pluripotent stem cells by contacting a somatic cell with a nuclear reprogramming substance and one or more of such proteins and nucleic acids that encode such proteins. The invention further provides an induced pluripotent stem cell that has an exogenous nucleic acid encoding such a protein, as well as agents for use in the aforesaid methods. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 12/37 |
| Publication No: | EP 9506039 B1 |
| Title: | Method for screening drugs for suppressing inflammasome activity |
| Publication Type: | United States Utility Patent |
| Publication Date: | January 4, 2017 |
| Filing Date: | November 17, 2011 |
| Inventors: | Tatsutoshi Nakahata, Megumu Saito, Takayuki Tanaka, Shinya Yamanaka |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a method for screening drugs for suppressing inflammasome activity, using macrophages derived from induced pluripotent stem cells (iPS cells) having mutant NLRP3 gene. |
| Representative Figure: | |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 13/37 |
| Publication No: | US 9637732 B2 |
| Title: | Method of efficiently establishing induced pluripotent stem cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | May 2, 2017 |
| Filing Date: | November 4, 2011 |
| Inventors: | Shinya Yamanaka, Kazutoshi Takahashi, Koji Tanabe, Hong Hyenjong |
| Assignee: | Kyoto University |
| Abstract: | Provided is a method of improving the efficiency of iPS cell establishment, comprising bringing one or more factors selected from the group consisting of proteins belonging to cyclin D family and nucleic acids that encode the same into contact with a somatic cell, in the step of nuclear reprogramming of the somatic cell. Also provided are a method of producing an iPS cell comprising the step of bringing the factor(s) and nuclear reprogramming substance(s) into contact with a somatic cell, an iPS cell comprising a nucleic acid that encodes a protein belonging to cyclin D family that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate. |
| Representative Figure: | |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 14/37 |
| Publication No: | US 9447408 B2 |
| Title: | Method of efficiently establishing induced pluripotent stem cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | September 20, 2016 |
| Filing Date: | September 14, 2011 |
| Inventors: | Shinya Yamanaka, Yoshinori Yoshida, Hidaka Yokota |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a method of improving the efficiency of establishment of induced pluripotent stem (iPS) cells by inhibiting p38 function in the step of somatic cell nuclear reprogramming. The 38 function can be inhibited by bringing an inhibitor selected from the group consisting of (1) a chemical inhibitor of p38 (2) a dominant negative mutant of p38 or a nucleic acid that encodes the same, (3) a nucleic acid selected from the group consisting of siRNAs and shRNAs targeted to p38 and DNAs that encode the same and (4) an inhibitor of p38 pathway into contact with a somatic cell and the like. The present invention also provides an agent for improving the efficiency of establishment of induced pluripotent stem cells, which contains an inhibitor of p38 function, particularly an inhibitor selected from the group consisting of (1) a chemical inhibitor of p38 (2) a dominant negative mutant of p38 or a nucleic acid that encodes the same, (3) a nucleic acid selected from the group consisting of siRNAs and shRNAs targeted to p38 and DNAs that encode the same and (4) an inhibitor of p38 pathway. Moreover, the present invention provides a production method of iPS cells, which includes bringing a nuclear reprogramming substance and an inhibitor of p38 function into contact with a somatic cell.td> |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 15/37 |
| Publication No: | US 8932857 B2 |
| Title: | Method for selecting reduced differentiation resistance human induced pluripotent stem cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | January 13, 2015 |
| Filing Date: | June 15, 2011 |
| Inventors: | Shinya Yamanaka, Kazutoshi Takahashi, Mari Ohnuki |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a method for selecting human induced pluripotent stem (iPS) cells which can be safely used for transplantation. That is, the present invention provides a method for selecting human iPS cells having reduced differentiation resistance, comprising the steps of: (1) inducing differentiation of human iPS cells; (2) detecting remaining undifferentiated cells after the step (1); and (3) selecting human iPS cells whose rate of remaining undifferentiated cells detected in step (2) is equivalent to or not more than that of control cells. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 16/37 |
| Publication No: | CA 2789749 C |
| Title: | Method of efficiently establishing induced pluripotent stem cells |
| Publication Type: | Canadian Patent |
| Publication Date: | September 4, 2018 |
| Filing Date: | February 16, 2011 |
| Inventors: | Shinya Yamanaka, Naoki Goshima, Momoko Maekawa, Yoshifumi Kawamura, Hiromi Mochizuki |
| Assignee: | Kyoto University |
| Abstract: | Provided are a method of improving the efficiency of establishment of iPS cells, comprising the step of contacting one or more substances selected from the group consisting of members of the GLIS family (e.g., GLIS1) and nucleic acids that encode the same and one or more substances selected from the group consisting of members of the Klf family and nucleic acids that encode the same, with a somatic cell, an iPS cell comprising an exogenous nucleic acid that encodes a member of the GLIS family or a member of the Klf family, that can be obtained by the method, and a method of producing a somatic cell by inducing the differentiation of the iPS cell. |
| Representative Figure: | |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 17/37 |
| Publication No: | US 9005967 B2 |
| Title: | Myc variants improve induced pluripotent stem cell generation efficiency |
| Publication Type: | United States Utility Patent |
| Publication Date: | April 14, 2015 |
| Filing Date: | anuary 21, 2011 |
| Inventors: | Shinya Yamanaka, Masato Nakagawa |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a method for improving iPS cell generation efficiency, which comprises a step of introducing a Myc variant having the following features: (1) having an activity to improve iPS cell generation efficiency which is comparative to, or greater than that of c-Myc; and (2) having a transformation activity which is lower than that of c-Myc; or a nucleic acid encoding the variant, in a nuclear reprogramming step. Also, the present invention provides a method for preparing iPS cells, which comprises a step of introducing the above Myc variant or a nucleic acid encoding the variant and a combination of nuclear reprogramming factors into somatic cells. Moreover, the present invention provides iPS cells comprising the nucleic acid encoding the Myc variant which can be obtained by the above method, and a method for preparing somatic cells which comprises inducing differentiation of the iPS cells. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 18/37 |
| Publication No: | EP 2524033 B1 |
| Title: | Method for screening induced pluripotent stem cells |
| Publication Type: | European Patent |
| Publication Date: | April 13, 2016 |
| Filing Date: | January 17, 2011 |
| Inventors: | Shinya Yamanaka, Michiyo Koyanagi |
| Assignee: | Kyoto University |
| Abstract: | The present invention relates to miRNA or genes expressed in induced pluripotent stem cells, and a method for screening for induced pluripotent stem cells having functions equivalent to those of embryonic stem cells by confirming methylation of specific gene regions of induced pluripotent stem cells. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 19/37 |
| Publication No: | EP 2480657 B1 |
| Title: | Method of efficiently establishing induced pluripotent stem cells |
| Publication Type: | European Patent |
| Publication Date: | January 17, 2018 |
| Filing Date: | September 22, 2010 |
| Inventors: | Shinya Yamanaka, Koji Tanabe |
| Assignee: | Kyoto University |
| Abstract: | Provided are a method of improving iPS cell establishment efficiency, comprising the step of transferring Lin28B or a nucleic acid that encodes Lin28B to a somatic cell, particularly to a somatic cell on which Lin28 is ineffective or less effective than Lin28B in improving iPS cell establishment efficiency, and a method of producing an iPS cell, comprising the step of transferring Lin28B or a nucleic acid that encodes Lin28B and a nuclear reprogramming substance to a somatic cell. Also provided are an iPS cell comprising a nucleic acid that encodes Lin28B, that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate into a somatic cell. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 20/37 |
| Publication No: | US 8871504 B2 |
| Title: | Method of selecting safe pluripotent stem cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | October 28, 2014 |
| Filing Date: | September 2, 2010 |
| Inventors: | Shinya Yamanaka, Mari Ohnuki |
| Assignee: | Kyoto Univesity |
| Abstract: | Provided is a method of selecting highly safe pluripotent stem cells that do not exhibit differentiation resistance, comprising the steps of (1) inducing a pluripotent stem cell to differentiate, (2) culturing the cell under conditions for maintaining undifferentiated state, (3) detecting the generation of an undifferentiated cell by the cultivation, and comparing the finding with a control, and (4) selecting a pluripotent stem cell whose detected value is not more than a control generation value. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 21/37 |
| Publication No: | US 9005976 B2 |
| Title: | Selection method of induced pluripotent stem cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | April 14, 2015 |
| Filing Date: | September 1, 2010 |
| Inventors: | Shinya Yamanaka, Takayuki Tanaka |
| Assignee: | Kyoto University |
| Abstract: | The present invention relates to a method for selecting induced pluripotent stem (iPS) cells. More particularly, the present invention provides: a method for selecting an iPS cell, comprising the steps of: (1a) measuring the expression level of an exogenous nuclear reprogramming gene(s) in a test iPS cell; and (2a) selecting an iPS cell in which the expression level(s) of an exogenous nuclear reprogramming gene(s) is/are less than or equal to the expression level(s) in control iPS cells; and a method for selecting an iPS cell, comprising the steps of: (1b) measuring the expression level of an exogenous nuclear reprogramming gene(s) and the sum the expression levels of the exogenous nuclear reprogramming gene(s) and the corresponding endogenous gene iPS cell; and (2b) selecting an iPS cell in which the ratio of the expression level of an exogenous nuclear reprogramming gene(s) relative to the sum of the expression levels of the exogenous transgene(s) and the corresponding endogenous gene(s) is less than 1 to the ratio in the control iPS cell. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 22/37 |
| Publication No: | EP 2438159 B1 |
| Title: | Method for selecting clone of induced pluripotent stem cells |
| Publication Type: | European Patent |
| Publication Date: | October 3, 2018 |
| Filing Date: | May 28, 2010 |
| Inventors: | Hideyuki Okano, Yohei Okada, Shinya Yamanaka, Kyoko Miura |
| Assignee: | Kyoto University |
| Abstract: | To efficiently identify and select a clone from clones of induced pluripotent stem cells (iPS cell) having low tumor formation rate in vivo when allowed to differentiate and transplanted in a living body, iPS cells of the clones are induced to differentiate, undifferentiated cells among the cells after the induction of differentiation are detected, and a clone having the content of the undifferentiated cell below a control is selected. |
| Representative Figure: | |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 23/37 |
| Publication No: | US 9045738 B2 |
| Title: | Method for producing induced pluripotent stem cells and method for culturing the same |
| Publication Type: | United States Utility Patent |
| Publication Date: | June 2, 2015 |
| Filing Date: | May 28, 2010 |
| Inventors: | Shinya Yamanaka, Kazutoshi Takahashi |
| Assignee: | Kyoto University |
| Abstract: | Induced pluripotent stem cells are produced from human somatic cells by co-culturing human somatic cells having a reprogrammed nucleus with human cells as feeder cells. Induced pluripotent stem cells are produced from somatic cells by co-culturing somatic cells having a reprogrammed nucleus with autologous cells as feeder cells. Induced pluripotent stem cells are cultured with culture supernatant of somatic cells. |
| Representative Figure: | |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 24/37 |
| Publication No: | US 8951801 B2 |
| Title: | Method for making IPS cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | February 10, 2015 |
| Filing Date: | February 19, 2010 |
| Inventors: | Shinya Yamanaka, Naoki Goshima, Momoko Maekawa, Yoshifumi Kawamura, Hiromi MOCHIZUKI |
| Assignee: | Kyoto University |
| Abstract: | Reprogramming substances capable of substituting for Klf4, selected from the group consisting of members of the IRX family (e.g., IRX6), members of the GLIS family (e.g., GLIS1), members of the PTX family (e.g., PITX2), DMRTB1, and nucleic acids that encode the same, are provided. Also provided are a method of producing iPS cells, comprising the step of introducing into a somatic cell both one or more kinds of the above-described nuclear reprogramming substances and a substance capable of inducing iPS cells from a somatic cell when combined with Klf4. Still also provided are iPS cells comprising an extraneous nucleic acid that encodes any one of the above-described nuclear reprogramming substances, that can be obtained by the method, and a method of producing somatic cells by inducing the iPS cells to differentiate. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 25/37 |
| Publication No: | US 8129187 B2 |
| Title: | Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2 |
| Publication Type: | United States Utility Patent |
| Publication Date: | March 6, 2012 |
| Filing Date: | February 18, 2012 |
| Inventors: | Shinya Yamanaka, Kazutoshi Takahashi, Keisuke Okita |
| Assignee: | Kyoto University |
| Abstract: | The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 26/37 |
| Publication No: | EP 2202309 B1 |
| Title: | Efficient method for nuclear reprogramming |
| Publication Type: | European Patent |
| Publication Date: | April 15, 2014 |
| Filing Date: | December 8, 2009 |
| Inventors: | Shinya Yamanaka, Michiyo Koyanagi |
| Assignee: | Kyoto University |
| Abstract: | This invention relates to a method for preparing an induced pluripotent stem cell from a somatic cell, comprising a step of nuclear reprogramming of the somatic cell with a nuclear reprogramming factor(s) in the presence of at least one miRNA, wherein the miRNA has a capavity to offer a higher nuclear reprogramming efficiency in the presence of the miRNA than in the absence of the miRNA, and wherein the nuclear reprogramming factor comprises at least (a) an Oct family member, (b) an Oct family member and a Klf family member, (c) an Oct family member and Nanog, or (d) an Oct family member, a Klf family member, and an Myc family member, but it does not comprise an Sox family member. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 27/37 |
| Publication No: | EP 2321406 B1 |
| Title: | Method for selecting secondary neurosphere derived from induced pluripotent stem cell |
| Publication Type: | European Patent |
| Publication Date: | December 10, 2014 |
| Filing Date: | August 5, 2009 |
| Inventors: | Hideyuki Okano, Osahiko Tsuji, Masaya Nakamura, Shinya Yamanaka, Kyoko Miura |
| Assignee: | Kyoto University |
| Abstract: | In order to provide a therapeutic agent for nerve injury which contains iPS-derived neural stem cells and has low or no risk of side effects, as well as a method for treating a nerve injury using the iPS cells, by efficiently establishing in vivo the iPS-derived neural stem having low or no risk of tumor formation, neurospheres are formed following formation of embryoid bodies from the iPS cells, and a clone whose ratio of cells in which the promoter of Nanog gene is activated is 0.01% or less is selected, and the clone is administered to a patient suffering from the nerve injury. |
| Representative Figure: | No Figure |
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| Details | Google Patents USPTO Patent Database |
| Publication: | 28/37 |
| Publication No: | CA 2697621 C |
| Title: | Method for selecting secondary neurosphere derived from induced pluripotent stem cell |
| Publication Type: | Canadian Patent |
| Publication Date: | January 17, 2017 |
| Filing Date: | July 30, 2009 |
| Inventors: | Shinya Yamanaka, Yoshinori Yoshida |
| Assignee: | Kyoto University |
| Abstract: | Provided is a method of improving the efficiency of establishment of induced pluripotent stem cells, comprising culturing somatic cells under hypoxic conditions in the step of nuclear reprogramming thereof. |
| Representative Figure: | No Figure |
| Family | |
| Details | Google Patents USPTO Patent Database |
| Publication: | 29/37 |
| Publication No: | EP 2288692 B1 |
| Title: | Method of efficiently establishing induced pluripotent stem cells |
| Publication Type: | European Patent |
| Publication Date: | August 2, 2017 |
| Filing Date: | June 26, 2009 |
| Inventors: | Shinya Yamanaka, Kazutoshi Takahashi, Keisuke Okita |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a method of improving the efficiency of establishment of induced pluripotent stem (iPS) cells, comprising inhibiting the p53 function in the step of somatic cell nuclear reprogramming. The inhibition of p53 function is achieved by bringing a substance selected from the group consisting of (1) chemical inhibitors of p53, (2) dominant negative mutants of p53 and nucleic acids that encode the same, (3) siRNAs and shRNAs against p53 and DNAs that encode the same, and (4) p53 pathway inhibitors, into contact with a somatic cell, and the like. The present invention also provides an agent for improving the efficiency of establishment of iPS cells, the agent comprising an inhibitor of p53 function, particularly (1) chemical inhibitors of p53, (2) dominant negative mutants of p53 and nucleic acids that encode the same, (3) siRNAs and shRNAs against p53 and DNAs that encode the same, and (4) p53 pathway inhibitors. The present invention further provides a method of producing an iPS cell, comprising bringing a nuclear reprogramming substance and an inhibitor of p53 function into contact with a somatic cell. |
| Representative Figure: | No Figure |
| Family | |
| Details | Google Patents USPTO Patent Database |
| Publication: | 30/37 |
| Publication No: | US 8058065 B2 |
| Title: | Oct3/4, Klf4, c-Myc and Sox2 produce induced pluripotent stem cells |
| Publication Type: | United States Utility Patent |
| Publication Date: | November 15, 2011 |
| Filing Date: | June 6, 2009 |
| Inventors: | Shinya Yamanaka, Kazutoshi Takahashi |
| Assignee: | Kyoto Univesity |
| Abstract: | The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells. |
| Representative Figure: | No Figure |
| Family | |
| Details | Google Patents USPTO Patent Database |
| Publication: | 31/37 |
| Publication No: | US 9683232 B2 |
| Title: | Efficient method for nuclear reprogramming |
| Publication Type: | United States Utility Patent |
| Publication Date: | June 20, 2017 |
| Filing Date: | February 25, 2009 |
| Inventors: | Shinya Yamanaka, Michiyo Koyanagi |
| Assignee: | Kyoto Univesity |
| Abstract: | This relates to a method of preparing induced pluripotent stem cells, comprising a nuclear reprogramming step with a nuclear reprogramming factor in the presence of miRNA, wherein said miRNA has a property of providing a higher nuclear reprogramming efficiency in the presence of said miRNA than in the absence thereof. |
| Representative Figure: | No Figure |
| Family | |
| Details | Google Patents USPTO Patent Database |
| Publication: | 32/37 |
| Publication No: | EP 2096169 B1 |
| Title: | Nuclear reprogramming method |
| Publication Type: | European Patent |
| Publication Date: | November 18, 2020 |
| Filing Date: | October 31, 2008 |
| Inventors: | Shinya Yamanaka, Kazutoshi Takahashi, Masato Nakagawa |
| Assignee: | Kyoto University |
| Abstract: | A process for effectively generating safe induced pluripotent stem cells from somatic cells, comprising the step of introducing the following three genes: Oct family gene, Klf family gene, and Sox family gene into somatic cells, or the step of introducing a combination of the following two genes: Oct family gene and Sox family gene or a combination of the following two genes: Oct family gene and Klf family gene, and at least one kind of genes selected from the following three genes: L-Myc, Sall1, and Sall4 into somatic cells. |
| Representative Figure: | No Figure |
| Family | |
| Details | Google Patents USPTO Patent Database |
| Publication: | 33/37 |
| Publication No: | ES 2843833 T3 |
| Title: | Nuclear reprogramming method |
| Publication Type: | Spanish Patent |
| Publication Date: | July 20, 2021 |
| Filing Date: | October 31, 2008 |
| Inventors: | Shinya Yamanaka, Kazutoshi Takahashi, Masato Nakagawa |
| Assignee: | Kyoto University |
| Abstract: | A process for generating induced pluripotent stem cells from somatic cells, comprising the step of introducing the following genes: Oct family gene, Klf family gene, Sox family gene, and L-Myc into somatic cells, which it further comprises the step of introducing Lin28 into somatic cells. |
| Representative Figure: | |
| Family | |
| Details | Google Patents USPTO Patent Database |
| Publication: | 34/37 |
| Publication No: | CA 2732401 C |
| Title: | Efficient method for establishing induced pluripotent stem cells |
| Publication Type: | Canadian Patent |
| Publication Date: | August 5, 2014 |
| Filing Date: | October 2, 2008 |
| Inventors: | Kenichi Tezuka, Toshiyuki Shibata, Takahiro Kunisada, Naritaka Tamaoki, Tomoko Takeda, Shinya Yamanaka, Kazutoshi Takahashi |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a method of producing induced pluripotent stem (iPS) cells, comprising bringing a nuclear reprogramming substance into contact with dental pulp stem cells. By using dental pulp stem cells as a source of somatic cells, the efficiency of establishment of human iPS cells by transfer of 3 or 4 factors can be improved dramatically. Additionally, dental pulp stem cells are easily available because they can be isolated and prepared from extracted wisdom teeth and teeth ex-tracted because of periodontal disease and the like, so that they can be used widely as a source of somatic cells for iPS cell banks. |
| Representative Figure: | |
| Family | |
| Details | Google Patents USPTO Patent Database |
| Publication: | 35/37 |
| Publication No: | CA 2658463 C |
| Title: | Efficient method for nuclear reprogramming |
| Publication Type: | Canadian Patent |
| Publication Date: | July 21, 2015 |
| Filing Date: | May 23, 2008 |
| Inventors: | Shinya Yamanaka, Michiyo Koyanagi |
| Assignee: | Kyoto University |
| Abstract: | A method of preparing induced pluripotent stem cells, comprising a nuclear reprogramming step using a nuclear reprogramming factor in the presence of miRNA, wherein said miRNA has a property of providing a higher nuclear reprogramming efficiency in the presence of said miRNA than in the absence thereof. |
| Representative Figure: | |
 | |
| Family | |
| Details | Google Patents USPTO Patent Database |
| Publication: | 36/37 |
| Publication No: | EP 1970446 B1 |
| Title: | Nuclear reprogramming factor |
| Publication Type: | European Patent |
| Publication Date: | August 3, 2011 |
| Filing Date: | December 6, 2006 |
| Inventors: | Shinya Yamanaka |
| Assignee: | Kyoto University |
| Abstract: | Disclosed is a means for inducing the reprogramming of a differentiated cell without using any embryo or ES cell and establish an inducible pluripotent stem cell having similar pluripotency and growing ability to those of an ES cell in a simple manner and with good reproductivity. As the means, a nuclear reprogramming factor for a somatic cell is provided, which comprise products of the following three genes: an Oct family gene; a Klf family gene; and an Myc family gene. |
| Representative Figure: | No Figure |
| Family | |
| Details | Google Patents USPTO Patent Database |
| Publication: | 37/37 |
| Publication No: | US 7964401 B2 |
| Title: | Screening method for somatic cell nuclear reprogramming substance affecting ECAT2 and ECAT3 |
| Publication Type: | United States Utility Patent |
| Publication Date: | June 21, 2011 |
| Filing Date: | February 16, 2005 |
| Inventors: | Shinya Yamanaka |
| Assignee: | Kyoto University |
| Abstract: | The present invention provides a screening method for somatic cell nuclear reprogramming substances, which comprises (a) a step for bringing into contact with each other a somatic cell comprising a gene wherein a marker gene is present at a position permitting expression control by the expression control region of an ECAT gene, and a test substance, and (b) a step following the aforementioned step (a), for determining the presence or absence of the emergence of cells expressing the marker gene, and selecting a test substance allowing the emergence of the cells as a somatic cell nuclear reprogramming substance candidate, and the like. |
| Representative Figure: | No Figure |
| Family | |
| Details | Google Patents USPTO Patent Database |
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Name: Shinya Yamanaka
Birth: 4 September 1962, Osaka, Japan
Residence: Kyoto University, Kyoto, Japan, Gladstone Institutes, San Francisco, CA, USA
Award: "for the discovery that mature cells can be reprogrammed to become pluripotent."
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